Fig. 1.

Klp98A controls the intracellular distribution of autophagosomes in Drosophila fat body cells. (A) Domains of Klp98A orthologs in Drosophila and human (KIF16B, isoform 2). The kinesin motor, forkhead-associated (FHA) and PX domains are highlighted in red, green and yellow, respectively. (B) Clonal expression of an RNAi construct (RNAi 1) targeting Klp98A in GFP-marked cells under starvation causes perinuclear accumulation and reduced number of Atg8-positive structures (in gray). (C) Representative images at the nuclear plane (proximal) and cell periphery (distal plane) of Atg8-positive vesicles (in gray) in cells overexpressing Klp98A in clones marked by GFP (green) after 4 h starvation. A magnification of the boxed region is shown in the lower panels. Nuclei are labeled with DAPI in blue. Scale bars: 10 µm.