Fig. 2.

Expanded proliferative and prehypertrophic zones, but a smaller hypertrophic zone, in Panx3-null mice. (A) The forelimbs and hindlimbs of newborn WT and Panx3^−/− mice stained with Alizarin Red and Alcian Blue. (B) Histology for growth plate of newborn mice. (a) H&E staining. (b) Quantifications of the lengths in each zone in cartilage areas. P, proliferative zone; PH, prehypertrophic zone; H, hypertrophic zone. (C) qPCR for mRNA expression of the chondrocyte marker genes using total RNA isolated from whole tibias. *P<0.05; **P<0.01 (Student's t-tests). Results represent mean±s.d. of three independent experiments.