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. 2016 Mar 1;129(5):943–956. doi: 10.1242/jcs.177410

Fig. 2.

Fig. 2.

Chlamydomonas cell lysates contain active PAM. (A) Schematic of subcellular fractionation protocol for Chlamydomonas. (B) PHM activity was detected in all fractions. (C) Microsomal PHM activity plotted as a function of pH. (D) Microsomal PHM activity was greatest at 2.5–5 µM copper. (E) The microsomal fraction displayed PAL activity that was inhibited by a divalent metal ion chelator (EDTA) or heat (5 min at 100°C). Data shown are mean±s.d. (n=2 or 3). Graphs are from one of two experiments, which yielded similar results.