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. 2016 Mar 1;129(5):898–911. doi: 10.1242/jcs.181180

Fig. 7.

Fig. 7.

α-Catulin plays a crucial role in the formation of AChR clusters. Different siRNAs were evaluated for their ability to deplete α-catulin expression by using RT-qPCR (A) and immunoblotting (B). Error bars represent s.d. ns, not significant. (C) Knockdown of α-catulin blocks the formation of AChR clusters. Laminin-cultured C2C12 myotubes were transfected with the siRNAs against the indicated genes and stained with α-bungarotoxin to visualize AChRs; scale bar: 50 μm. (D) Quantification of the number of clusters per visual field in myotubes transfected with the indicated siRNAs. The percentage of clusters (normalized to that in control) is shown as an average from three independent experiments; the number of complex clusters was n=158 for untransfected control, n=158 for negative control, n=2 for positive control, n=1 for α-catulin siRNA 1, n=0 for siRNA 2, n=7 for siRNA 3. Two-tailed Student t-test, mean±s.d., *P<0.05, **P<0.01, ****P<0.0001. neg. contr., negative control, non-targeting siRNA; MuSK siRNA, positive control.