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. 2016 Mar 1;129(5):1072–1082. doi: 10.1242/jcs.181743

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Sustained actin polymerization is not required to maintain a pH gradient in the lysosomal synapse. (A) Ratiometric confocal images of J774 cells interacting with agLDL dual labeled with a pH-sensitive and a pH-insensitive fluorophore for 30 min. (B) Addition of 1 μM LatA for 30 min did not abolish the low pH regions. (C–H) J774 cells were treated with agLDL for 30 min (C–E) or 30 min followed by 30 min incubation with 1 μM LatA (F–H) followed by fixation in 3% PFA and staining F-actin with Alexa488–phalloidin. Using the same treatment conditions as in B, F-actin structures at the lysosomal synapse (arrows in C−E) were completely abolished.