Skip to main content
. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Eur J Immunol. 2015 Aug 28;45(11):3052–3063. doi: 10.1002/eji.201545696

Figure 2. IL-33 rebalances IL-17/IFN-γ-producing γδ T cells in the livers of early LCMV-infected mice.

Figure 2

(A) WT and IL-33−/− mice were infected and sacrificed at 16 hours post-infection (hpi). IHLs were isolated for FACS analysis. CD3+γδ TCR + cells were first gated, which was followed by the detection of intracellular IL-17 and IFN-γ levels. The absolute numbers of hepatic IL-17- and IFN-γ-producing γδ T cells are also shown (right). Data are shown as mean + SEM of 3–4 mice/group from a single experiment representative of three experiments performed. (B) The hepatic IL-17- and IFN-γ-producing γδ T cells were gated for examining the surface CD27 expression. (C) The infected WT mice were i.p.-treated with PBS and rIL-33 (1 μg/mouse) respectively at 2 hpi, and sacrificed at 16 hpi. The hepatic IL-17- and IFN-γ-producing γδ T cells were analyzed. (D) Surface ST2 expression on γδ T cells. The data are from a single experiment representative of at least three experiments performed. One-way ANOVA was used for statistical analysis. * P<0.05; ** P<0.01.