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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Eur J Immunol. 2015 Aug 28;45(11):3052–3063. doi: 10.1002/eji.201545696

Figure 6. Surface expression intensities of DC costimulatory molecules are not altered by IL-33.

Figure 6

(A) WT and IL-33−/− mice were infected for 3 days and IHLs were isolated and stimulated with LPS overnight. GolgiStop was added at the last 4 h. Intracellular IL-12 and surface CD80, CD86 and MHCII were detected on CD11b+CD11c+ cells by flow cytometry. (B) BMDCs were cultured with or without virus and rIL-33 for 2 days. Surface CD80, CD86, OX40L and MHCII were detected. The data are from a single representative experiment. The experiment was repeated three times independently, and representative graphs are shown (n = 3–5 mice/group).