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. Author manuscript; available in PMC: 2016 Aug 2.
Published in final edited form as: Expert Rev Mol Diagn. 2015 Aug 2;15(10):1293–1310. doi: 10.1586/14737159.2015.1071666

Figure 1.

Figure 1

Characteristics of exosomes isolated from the supernatant of Kasumi-1 (AML) cell line using differential centrifugation, ultrafiltration, size-exclusion chromatography and ultracentrifugation were studied. In A, following ultracentrifugation, exosomes were floated on a continuous sucrose density gradient, and the collected gradient fractions were tested by Western blots for CD34 and CD81. CD34+ and CD81+ exosomes were recovered at the sucrose density of 1.10–1.14 g/mL. In B, transmission electron microscopy of negatively-stained Kasumi-1 exosomes. In C, Western blots of Kasumi-1 exosomes (Kas) and exosomes isolated from plasma of a normal donor (NC). Each lane was loaded with 10µg exosomal protein. Reproduced from Hong CS, Muller L, Boyiadzis M, Whiteside TL, Isolation and characterization of CD34+ blast-derived exosomes in acute myeloid leukemia. PLoS One, 9(8), e103310 (2014) [80].

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