Fig. 3.

CSF-cNs are Gata2⁺ late-born spinal cells. (A-D) Pkd2l1 expression in E14.5, E15.5 and P0 spinal cord sections and quantification of Pkd2l1⁺ cells (n=3-10 animals each). Arrowheads indicate Pkd2l1⁺ cells. Letters indicate statistical significance (P<0.001, Kruskal–Wallis with post-hoc Dunn's test) (groups with different letters are significantly different from one another). (E-H) Pkd2l1⁺ cells (1389/1409) express Gata2 at E14.5 and P0, as shown by immunohistochemistry (E,F) or in Gata2^GFP mice (G,H). (I-K) Birthdate analysis of CSF-cNs. BrdU was applied for 24 h (pulsed every 4 h) during E13-E14 (I,I′) or E16-E17 (J,J′), and analyzed at P0. Arrows indicate Brdu⁺Pkd2l1⁺ cells. (K) Percentage of Pkd2l1⁺ cells labeled with BrdU (27 sections, 3 pups each). (L-N) CSF-cNs arise along with glial cells. At E14.5, when Pkd2l1⁺Gata2⁺ CSF-cNs begin to appear (arrowheads, insets), astrocyte precursors outside the ventricular zone (dashed line) are identified by Sox2 and Sox9 (L) or Nfia and Sox9 (M) expression. Migrating oligodendrocytes were identified by Olig2 (N). Bars are mean±s.d. Scale bars: 30 µm in A-C,I,J,L-N; 10 µm in E-H,I′,J′. See also Fig. S3. Gray line indicates position of the ventricle or the central canal in A-C,E-H,I-J′.