Skip to main content
. 2016 Mar 1;143(5):841–850. doi: 10.1242/dev.129320

Fig. 5.

Fig. 5.

GRG localizes to the Atoh1 locus in a Notch-dependent manner in neural progenitor cells. Neural progenitor cells (NPCs) were differentiated from ESCs and FACS purified (Sox1-GFP+). (A) Atoh1 and Hes5 mRNA expression levels in 46C ESCs and NPCs that were treated with control (DMSO) or γ-secretase inhibitor (DAPT) for 48 h. Similar to supporting cells, inhibition of Notch signaling by DAPT induces the expression of Atoh1 and represses the expression of Hes5 in NPCs. n=4. (B) HES5 localizes to the proximal promoter and not the enhancer by ChIP-qPCR of NPCs transfected with CMV-FLAG-Hes5 plasmid (or CMV-RFP control). Results are reported as fold enrichment (FLAG-Hes5 transfected percentage input/RFP transfected percentage input). n=3. (C) GRG localizes to the Atoh1 promoter in a Notch-dependent manner. ChIP-qPCR with anti-pan-GRG antibody and primers scanning the Atoh1 locus in NPCs treated with control (DMSO) or γ-secretase inhibitor (DAPT) for 24 h after 7 days of differentiation. n=3. (D) Schematic of the Atoh1 locus in mouse showing the promoter and the autoregulatory enhancer regions. Numbers refer to the regions amplified in ChIP-qPCR. The primers used to measure the enrichment after ChIP are shown by arrows. All values are mean±s.e.m. *P<0.05, **P<0.005. See also Figs S3-S5.