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. 2015 Nov 17;173(1):115–127. doi: 10.1111/bph.13341

Figure 1.

Figure 1

NADA does not activate K channels in AtT20 cells. GIRK activation was determined as described in the Methods. (A) Raw traces showing changes in fluorescent signal from the membrane potential dye (raw fluorescence units, RFU) in AtT20‐rCB1 cells during application of CP55940 but not NADA. Drug was added for the duration of the bar; the traces are representative of at least five independent experiments. (B) Concentration‐response curves for CP55940 activation of GIRK in AtT20‐rCB1 cells in the presence and absence of 30 μM NADA. Data represent the mean ± SEM of at least five independent experiments performed in duplicate; pooled data were fit with a four‐parameter logistic equation. There was no difference in the potency or maximal effect of CP55940 between control conditions or in the presence of NADA.