Figure 3.

NADA does not modulate ERK phosphorylation via CB₁ receptors in HEK 293‐hCB₁ cells. ERK1/2 phosphorylation was determined as outlined in the Methods. The time plot represents the fluorescent signal arising from phosphorylated ERK1/2 measured every minute after drug addition (T = 0). CP55940 (P = 0.0.33) but not NADA (P = 0.503) produced a significant increase in ERK1/2 phosphorylation compared with vehicle at 5 min. The data presented are the mean ± SEM of four experiments, each performed in duplicate.