Figure 5.

NADA elevates [Ca]_i in a manner consistent with activation of G_q. Changes in [Ca]_i were measured as described in the Methods. (A) Traces from a representative experiment showing that elevations of [Ca]_i were inhibited by U73122, but not affected by pretreatment with pertussis toxin overnight. (B) Summary of the data from five similar experiments, expressed as mean ± SEM. (C) Traces from a representative experiment showing that elevations of [Ca]_i were attenuated by a peptide antagonist of G_q (palpeptide) but not by the scrambled palpeptide control. (D) Summary of the data from five similar experiments, the G_q palpeptide significantly inhibited the elevations of [Ca]_i in response to 30 μM NADA, (P = 0.006).