Changes in hyaloid‐retinal vessel diameter induced by 130 mM glucose(HG). (A) The average diameter and fluorescent images of hyaloid‐retinal vessels in HG‐treated larvae. The diameter of hyaloid‐retinal vessels was measured at locations proximal (red circle) to the OD from each group. The graph displays the mean AU for vessel diameter. The vessel diameter of each lens was measured three times. ***P < 0.001, significantly different from control, n = 8–10 embryos per group. Scale bar = 40 µm. (B) Inductivity for the vessel diameter was increased in the HG‐treated group, compared with the control group. ***P < 0.001, significantly different from control, n = 8–10 embryos per group. (C) Survival rates in HG‐treated zebrafish larvae. *P < 0.05, significantly different from control, n = 10 embryos per group. (D) After HG‐treatment for 3 days, the zebrafish displayed increased levels of glucose. ***P < 0.001, significantly different from control, n = 20 embryos per group. (E) Control and HG‐treated larvae did not show gross morphological changes at 6dpf. Scale bar = 300 µm.