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. 2015 Dec 1;173(1):212–221. doi: 10.1111/bph.13359

Figure 2.

Figure 2

Identification of myosin‐9 as a HHT target protein. (A) Silver‐stained gel and (B) colloidal blue‐stained gel loaded with lysates of Kasumi‐1 cells eluted from biotin‐conjugated (control, lane 1) and HHT‐conjugated (lane 2) affinity columns. Two proteins with molecular masses of approximately 220 and 40 KDa were detected in the eluates. (C) The two bands were confirmed to be myosin‐9 and actin by immunoblot analysis with the corresponding antibodies. (D) HHT directly binds to the myosin‐9 protein. Purified GST‐myosin‐9 and GST proteins were individually incubated with biotinylated HHT, and the complexes were isolated with avidin agarose. Immunoblot analysis showed that HHT pulled down myosin‐9 protein but not GST protein.