TABLE 1.
Toxoplasma strains used in this study
| Strain | Parental strain | Genotype | Gene targeted | Selection marker | Selection drug | Ref. |
|---|---|---|---|---|---|---|
| KU80ΔΔ | RH(I) | Δku80;Δhxgprt | 26 | |||
| RHΔphyA-1 | KU80ΔΔ | phyAΔ;Δku80 | phyA-exon 1 | Hxgprt | MPA, xanthine | 5 |
| RHΔphyA-2 | KU80ΔΔ | phyAΔ;Δku80 | phyA-exons 1–9 (all) | Hxgprt | MPA, xanthine | TRa |
| RHΔgnt1 | KU80ΔΔ | gnt1Δ;Δku80 | gnt1-exon 1 (all) | Hxgprt | MPA, xanthine | TR |
| RHΔgnt1/complemented | RHΔgnt1 | Δku80;Δhxgprt | ΔHxgprt | 6-Thioxanthine | TR | |
| RHΔpgtA | KU80ΔΔ | pgtAΔ;Δku80 | pgtA-exons 1–14 (all) | Hxgprt | MPA, xanthine | TR |
| RHΔpgtA/complemented | RHΔpgtA | Δku80;Δhxgprt | ΔHxgprt | 6-Thioxanthine | TR | |
| RHΔΔ/Skp1-SF | KU80ΔΔ | Skp1SF;Δku80; Δhxgprt;CAT+ | Skp1 C-terminus | CAT | Chloramphenicol | TR |
| RHΔphyA-1/Skp1-SF | RHphyAΔ-1 | Skp1SF;phyAΔ; Δku80;CAT+ | phyA-exon 1; Skp1 C terminus | CAT | Chloramphenicol | TR |
a TR means this report.