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. 2016 Jan 5;291(9):4282–4293. doi: 10.1074/jbc.M115.701094

FIGURE 6.

FIGURE 6.

CIDE proteins promote lipid storage in ob/ob hepatocytes. A, expression levels of CIDEs in the livers of wild-type (WT) and ob/ob mice. B, representative image showing the lipid droplet morphology in wild-type and ob/ob hepatocytes. Scale bar, 10 μm. C, Cidea and Cideb localized on LDCSs. ob/ob hepatocytes were stained with antibodies against Cidea (green) and Cideb (red). Scale bar, 10 μm. D, Cidec and Cideb localized on LDCSs. ob/ob hepatocytes were stained with antibodies against Cidec (green) and Cideb (red). Scale bar, 10 μm. Arrowheads point to LDCSs. E, expression levels of the indicated proteins. Quantitation of the bands was performed using Quantity One software and are expressed as the fold change, after correction for actin levels. Values are averages obtained from three experiments. F, representative image showing the LD morphology after the depletion of different proteins. Scale bar, 10 μm. G, hepatocytes TAG level (n = 4). H, lipid exchange rates (n = 4). Quantitative data are presented as the mean ± S.E. Differences were considered significant at p < 0.05. *, p < 0.05; ***, p < 0.001; NS, no significant difference.