FIGURE 1.

Topologies of the plasmid constructs used in these studies. A, Sleeping Beauty plasmids used for integration of the expression cassettes. B, pRRL plasmids used for lentivirus generation. IR-L and IR-R, left and right inverse repeats of SB. LTR-5′ and LTR-3′, 5′ and 3′ long terminal repeats. CMV, CMV intermediate early promoter. CAG, CMV early enhancer/chicken β-actin promoter. EF1, elongation factor-1 promoter. PuroR, puromycin resistance gene. PrP-wt, wild type mouse prion protein. PrPΔCR, mouse prion protein missing the central region (aa 105–125). Sho, mouse Shadoo protein.