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. 2015 Dec 31;291(9):4473–4486. doi: 10.1074/jbc.M115.679035

FIGURE 3.

FIGURE 3.

Coexpression of PrP-WT but not Sho diminishes PrPΔCR caused Zeocin hypersensitivity. A and B, PrPΔCR makes SH-SY5Y cells hypersensitive to Zeocin. Shown are cytotoxicity assays, using MTT reagent. A, representative experiment carried out at Zeocin concentrations between 0 and 100 μg/ml on various cells, as indicated, for 48 h. Values are means ± S.D. (error bars) of replicas in the individual experiment. B, bars show the means ± S.D. of cell viabilities measured at 6.25 μg/ml Zeocin concentration in n = 4 independent experiments. C and D, coexpression of PrP-WT but not Sho diminishes PrPΔCR-caused Zeocin hypersensitivity. Shown is a cytotoxicity assay, using PrestoBlue reagent. C, representative experiment carried out at Zeocin concentrations between 0 and 100 μg/ml Zeocin on various cells as indicated for 48 h. Values are means ± S.D. of replicas. D, bars show the means ± S.D. of cell viabilities measured at 6.25 μg/ml Zeocin concentration in n = 3 independent experiments. A–D, 100% is the absorbance (A and B) or fluorescence (C and D) value of untreated cells of each cell type. B and D, samples were compared with the leftmost values on each diagram (B, SH-SY5Y; D, SH/ΔCR); *, p = 0.04; ***, p < 0.001.