. 2015 Dec 31;291(9):4473–4486. doi: 10.1074/jbc.M115.679035

TABLE 1.

The DNA oligonucleotides used in the cloning processes

Oligonucleotide name	DNA sequence
Delta105–125for	`AACAAGCCCAGCAAACCAGGCTACATGCTGGGGAG`
Delta105–125rev	`CTCCCCAGCATGTAGCCTGGTTTGCTGGGCTTGTT`
XhoImutator5	`CGGCCGCGGAGGCGCACGAGGCAGTGCCCGG`
XhoImutator3	`CCGGGCACTGCCTCGTGCGCCTCCGCGGCCG`
ApoICMV5	`CGCGCGAAATTTCTGCTTCGCGATGTACGGG`
ApoIBGH3	`GGCCCGAAATTTCCACCGCATCCCCAGCATG`
PrPNheI5	`GCCGGGCTAGCCACCATGGCGAACCTTGGCTACTG`
PrPBamHI3	`TACCACGGATCCTCATCCCACGATCAGGAAGATG`
mShoBamHI5	`CCCGAAGGATCCGCCACCATGAACTGGACTGCTGCCACG`
XhoISho3	`GGCGCGCTCGAGCTAAGGCCGAAGCAGTTCTAG`
Linker2-3048-fwd	`GTACAAGTGAGGGCGCGCCAAACATATGAAACGTACGAA`
Linker2-3048-rev	`TCGATTCGTACGTTTCATATGTTTGGCGCGCCCTCACTT`
V-CMV-PrP fw	`TTTGCAGGCGCGCCCGATGTACGGGCCAGATATACG`
V-CMV-PrP rev	`CACTATTGTACAGGGCCCTCTAGATGCATGCTCGAGC`
V-CMV-PA rev	`ATAGAGTGTACAACATCCCCAGCATGCCTGC`
Sho-epi1-fwd	`GTACAGGCTCTGGCTGGAGGAGGACCTCAGGGCCTGGAGAGCTA`
Sho-epi1-rev^a	`AGGCCCTGAGGTCCTCCTCCAGCCAGAGCCT`
Sho-epi2-fwd^a	`GGCCTGGAGGACGATGAGAATGGGGCAATGGGAGGC`
Sho-epi2-rev^a	`TGCCCCATTCTCATCGTCCTCCAGGCCTAGCTCTCC`
Sho-epi3-fwd^a	`AACGGAACCGACCGAGGAGTCTACAGCTACA`
Sho-epi3-rev	`GTACTGTAGCTGTAGACTCCTCGGTCGGTTCCGTTGCCTCCCAT`

^a These oligonucleotides are 5′-phosphorylated.