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Peptide ligands without Arg or Lys can induce full headpiece opening in Mg2+/Ca2+. A, Stokes radii measured by DLS (data measured in triplicate, mean ± S.D.) and size exclusion chromatography (Superdex 200, values measured in duplicate, mean and difference from mean). nd = not determined. B, overlaid Superdex 200 chromatograms of unclasped αIIbβ3 headpiece in the absence (green) or presence of 1 mm RGDF peptide (purple) or 1 mm AGDV peptide (blue).
