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. 2015 Dec 14;291(9):4561–4579. doi: 10.1074/jbc.M115.677898

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FIGURE 7. — Whole cell patch clamp analysis of NCX1 regulation by PLM and PP1c. HEK293 cells were transfected with WT NCX1(FL), WT NCX1(FL), and PLM or Pro substitution mutant NCX1(F407P) and PLM. NCX1 current was elicited by a descending voltage ramp from 120 to −100 mV. Current traces of WT NCX1(FL) (n = 9) (A), NCX1(F407P) (n = 7) (B), WT NCX1(FL) and PLM (n = 6) (C), and NCX1(F407P) and PLM (n = 5) (D) are shown. E and F, relationship between current (normalized to cell capacitance) and membrane voltage (V_m), presented for potentials between +80 and −80 mV. *, p < 0.05 (WT NCX1(FL) + PLM versus WT NCX1(FL) and NCX1(F407P) + PLM versus WT NCX1(FL)). #, p < 0.05 (NCX1(F407P) + PLM versus WT NCX1(FL) + PLM (n = 5)).