FIGURE 3.

p120RasGAP associates with a DCC multiprotein complex in netrin-1-stimulated cortical neurons. A, cortical neurons were stimulated with netrin-1 for 10 min. p120RasGAP was immunoprecipitated from cell lysates with anti-p120RasGAP antibodies or mouse IgGs as a control. Immunoprecipitated proteins and total cell lysates (TCL) were resolved by SDS-PAGE and immunoblotted (IB) with antibodies against the indicated proteins (pY (RasGAP), anti-phosphotyrosine antibodies). Data were taken from the same film exposure. The white line between the RasGAP immunoprecipitation (IP) and IgG lanes indicates that irrelevant lanes were removed digitally from the original image. B, quantitative densitometry (mean ± S.E.) of Tyr(P) (the RasGAP)/RasGAP ratio is represented as the -fold change relative to 0 min of netrin-1 stimulation for at least three independent experiments; unpaired Student's t test; *, p < 0.05).