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Conditions to package virus
| Envelope coding vector pMD2.G (GP-VSVG) | Envelope coding vector pHCMV (GP-LCMV) | Lentiviral vector pLKO1.1 enhanced GFP | Packaging vector pCMV8.74 | Envelope coding vector/total transfected DNA (final %) | Titer of lentivirus (108 IU/ml) |
|---|---|---|---|---|---|
| μg | μg | μg | μg | ||
| 5 | 10 | 10 | 20 | 8.9 | |
| 1 | 10 | 10 | 5 | 12.6 | |
| 0.2 | 10 | 10 | 1 | 9.9 | |
| 5 | 10 | 10 | 20 | 7.9 | |
| 1 | 10 | 10 | 5 | 5.9 | |
| 0.2 | 10 | 10 | 1 | 2.8 |
