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Cross-linking of ΔN6-β2m and β2m. A, ΔN6-β2m and β2m were cross-linked with a 1:1 mixture of BS3-d0/d4. The gels show the presence of monomers, dimers, trimers, and tetramers. B, cross-links were verified by the presence of peak pairs in the mass spectra corresponding to light (d0) and heavy (d4) cross-linked peptides. C, cross-linked dipeptides containing the same or overlapping peptide sequences are unambiguously assigned to interprotein cross-links. An example spectrum is shown.
