TABLE 1.
List of primers using for mutations
| Name of primers | Forward sequence | Reverse sequence |
|---|---|---|
| K13R | CCCCAGCTATCGGAGGGCCACACTGTTTG | CAAACAGTGTGGCCCTCCGATAGCTGGGG |
| K34R | CGTGCAGAACAGCAGGAACGCCAAGGACA | TGTCCTTGGCGTTCCTGCTGTTCTGCACG |
| K34R/K37R | CAGCAGGAACGCCAGGGACAAGAACCTGA | TCAGGTTCTTGTCCCTGGCGTTCCTGCTG |
| K37R/K39R | GAACGCCAGGGACAGGAACCTGAAGCGGC | GCCGCTTCAGGTTCCTGTCCCTGGCGTTC |
| K39R/K42R | GGACAGGAACCTGAGGCGGCACTCCATCA | TGATGGAGTGCCGCCTCAGGTTCCTGTCC |
| K53R | GGTGCTGCCTTGGAGGAGGATCGTGGCGG | CCGCCACGATCCTCCTCCAAGGCAGCACC |
| K61R/K62R/K63R | GGTGTCAGCGAGGAGGAGGAACTCCAGGA | TCCTGGAGTTCCTCCTCCTCGCTGACACC |
| K66R/K67R | GAAGAAGAACTCCAGGAGGGCGCAGCCCA | TGGGCTGCGCCCTCCTGGAGTTCTTCTTC |
| K87R/K88R | CAATGAGAACCTGAGGAGGTCGCTGTCCT | AGGACAGCGACCTCCTCAGGTTCTCATTG |
| K126R/K127R | CTCCTCTTCTGTCAGGAGGGCCCCGCACC | GGTGCGGGGCCCTCCTGACAGAAGAGGAG |
| K140R | TGCAGGGACACCCAGACGGGTCATCGTCC | GGACGATGACCCGTCTGGGTGTCCCTGCA |
| K167R | GTGCTACCGCCTGAGGCACTTGTCCCCAA | TTGGGGACAAGTGCCTCAGGCGGTAGCAC |
| K246R | CTCCTACCCGCTCAGGCCCTTCCTGGTAG | CTACCAGGAAGGGCCTGAGCGGGTAGGAG |
| K254R | GGTAGAGAGCTGTAGGGAAGCCTTTTGGG | CCCAAAAGGCTTCCCTACAGCTCTCTACC |
| K271R | CCTCATGAGCTCCAGGATGCTGCAGATCA | TGATCTGCAGCATCCTGGAGCTCATGAGG |
| K290R | GTTCTCTGACTTGAGGAATGAGAGCGGTC | GACCGCTCTCATTCCTCAAGTCAGAGAAC |
| K298R/K299R | CGGTCAGGAGGACAGGAGGCGACTCCTCCTGG | CCAGGAGGAGTCGCCTCCTGTCCTCCTGACCG |
| MycKtoR | GGATCGGGAACAAAGACTCATCTCAGAAG | CTTCTGAGATGAGTCTTTGTTCCCGATCC |
| R126K/R127K | CTCCTCTTCTGTCAAGAAGGCCCCGCACC | GGTGCGGGGCCTTCTTGACAGAAGAGGAG |
| R167K | GTGCTACCGCCTGAAGCACTTGTCCCCAA | TTGGGGACAAGTGCTTCAGGCGGTAGCAC |
| R298K/R299K | CGGTCAGGAGGACAAGAAGCGACTCCTCC | GGAGGAGTCGCTTCTTGTCCTCCTGACCG |
| P247A | TACCCGCTCAAGGCCTTCCTGGTAGAGA | TCTCTACCAGGAAGGCCTTGAGCGGGTA |
| 23R-P247A | TACCCGCTCAGGGCCTTCCTGGTAGAGA | TCTCTACCAGGAAGGCCCTGAGCGGGTA |