FIGURE 11.
Effect of light on aspartate. A, ratios of Asp/Glu from retinas isolated from mouse eyes. The ratios of Asp and Glu were calculated from the experiment shown in Fig. 9. B, ratios of Asp/Glu from retinas incubated in culture dishes in the dark or light. The ratios of total Asp and Glu were calculated from the results of the experiment shown in Fig. 7. C, more aspartate is released in the dark than in light from retinas (n = 3). Dark-adapted mouse retinas were isolated and cultured either in the dark or in light in media with 5 mm glutamine. After 60 min the media were harvested, and metabolites were quantified by GC-MS (n = 3; error bars show S.D.).