FIGURE 10.
Palmitoylation is required for full gB fusion activity. A, ARPE cells were transfected with the indicated relative amounts of gB- or gBC777A-coding mRNAs, along with gH pentamer subunits and T7 RNA polymerase mRNAs; the luciferase-reporter fusion assay was carried out as in Fig. 8B in the presence (light gray) or absence (dark gray) of 50 μm 2Br-palmitate. Error bars indicate mean ± S.D. B, ARPE cells were co-transfected with gH pentamer and the indicated relative amounts of gB- or gBC777A-coding mRNAs. Cell nuclei were stained with DAPI; multinucleated cells are indicated by white arrows.