FIGURE 4.

Palmitoylation is required for efficient HCMV gB targeting to Pr55^Gag VLPs. A, anti-gB and -p24 immunoblots (IB) on total cell extracts from cells expressing Pr55^Gag together with gB or gB_C777A. For all samples, 10⁵ cell equivalents were loaded for blotting. B, immunoblot on VLP fractions obtained from cell cultures in A after mock or MβCD treatment and wash-out. Equal loading was as in Fig. 1B. C, particle titers (left panel) and anti-gB or -p24 immunoblots (right panel) on Pr55^Gag VLPs from cells co-expressing gB or gB_C777A treated or mock-treated (dark gray and light gray in the left panel, respectively) with 50 μm 2Br-palmitate (2Br-p). Equal loading was as above. Error bars indicate mean ± S.D.