FIGURE 5.

Palmitoylation-dependent gB partition with lipid rafts in human cells. ARPE cells were transfected with mRNAs coding for gB or gB_C777A and processed for raft flotation as described under “Experimental Procedures.” After ultracentrifugation, the density gradients were collected in 850-μl fractions, and the first 12 from the tube top were probed in immunoblot (IB) for gB, flotillin 1 (flt1), or the transferrin receptor (TfR). Cells were cholesterol-depleted with 10 mm MβCD (middle panel) or incubated with 50 μm 2Br-p (lower panel) as described in the legend for Fig. 4. Equal loading was as above.