TABLE 1.

Primer list; restriction sites (underlined), start/stop (lowercase) and mutated (boldface) codons, T7 promoter/terminator (italics), and overlapping sequences (underlined boldface) are indicated

Name	Sequence
OFw	5′-TATAAATATAGGATCCCGCCACCatgGAATCCAGGATCTGGTGCCTG-3′
mutFur	5′-GGTGTGAACTCCACGCGTGCAACCAAGGCTAGTACGGGCAATACGACCACCCT-3′
C777A	5′-CTATACTCGACAGCGGCGTCTCGCCATGCAGCCGCTGCAAAACCTCTTT-3′
ORv	5′-GCCGCGGTACCTCGAGttaCTTTTCGAATTGCGGATGC-3′
R7BFw	5′-TAATACGACTCACTATAGGGAGAatgGAATCCAGGATCTGGTGCCTG-3′
R7BRv	5′-tcaGACGTTCTCTTCTTCGTCAGAG-3′
R7HFw	5′-TAATACGACTCACTATAGGGAGAatgCGGCCCGGCCTCCCCTTC-3′
R7HRv	5′-tcaGCATGTCTTGAGCATGCGGTA-3′
R7LFw	5′-TAATACGACTCACTATAGGGAGAatgTGCCGCCGCCCGGATTGC-3′
R7LRv	5′-ttaGCGAGCATCCACTGCTTGAGG-3′
R71Fw	5′-TAATACGACTCACTATAGGGAGAatgCGGCTGTGTCGGGTGTGGC-3′
R71Rv	5′-ctaGTTGGCAAAGAGCCGCACGC-3′
R70Fw	5′-TAATACGACTCACTATAGGGAGAatgCTACGGCTTCTGCTTCGTCAC-3′
R70Rv	5′-tcaAACGATGAGATTGGGATGGGTG-3′
R78Fw	5′-TAATACGACTCACTATAGGGAGAatgAGTCCCAAAAACCTGACGCCG-3′
R78Rv	5′-tcaCTGCAGCATATAGCCCATTTTAG-3′
R7T7Fw	5′-TAATACGACTCACTATAGGGAGAatgAACACGATTAACATCGCTAAGAAC-3′
R7T7Rv	5′-ttaCGCGAACGCGAAGTCCGACT-3′
D7iLF1	5′-TAATACGACTCACTATAGGGAGAGGTACCGCGGGCCCGGGATC-3′
D7iLR1	5′-CTTCTTAATGTTTTTGGCATCTTCCATTATTATCATCGTGTTTTTCAAAGGAAAAC-3′
D7iLF2	3′-GTTTTCCTTTGAAAAACACGATGATAATAATGGAAGATGCCAAAAACATTAAGAAG-3′
D7iLR2	5′-AGAGGCCCCAAGGGGTTATGCTAttaCACGGCGATCTTGCCGCCCTTCT-3′
D7iLR2b	5′-CAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTA-3′