FIGURE 3.

PDI protein is heavily sulfenilated in the lungs of murine smokers. A, lungs of one-time smoker mice at different times post-exposure and lungs of chronic smokers exposed to smoke at different ages were harvested, and sulfenilated proteins were immunoprecipitated from total lung lysates and separated at non-reducing conditions. Western blots were probed for the presence of PDI among sulfenilated protein. Please note that sulfenilated PDI was observed to be predominantly present in HMWCs, which contain protein complexes with size over 108 kDa. B, graph represents quantification of sulfenilated PDI in controls and smokers of different ages. Relative quantitation of one-time and age-matched controls was performed by running one of acute controls and one of acute smokers on each gel.