FIGURE 1.

COX-2 regulated RPMI-8226 and U-266 cell proliferation. A, the protein levels of COX-1 and COX-2 in human umbilical vein endothelial cells, RPMI-8226 cells, and U-266 cells transfected or non-transfected with scramble, COX-1 siRNA, or COX-2 siRNA were measured by Western blotting. B–E, CCK-8 cell proliferation assays were performed to detect the effect of COX-1/2 inhibitors on proliferation of two MM cell lines. RPMI-8226 and U-266 cells were treated with graded concentrations of SC-560 (B and C) and NS-398 (D and E) for 24, 48, 72, and 96 h, respectively. Red, vehicle; green, 1 μm inhibitor; blue, 10 μm inhibitor; black, 30 μm inhibitor. *, p < 0.05 versus vehicle; #, p < 0.05, represents the differences among drug additional treatments with graded concentrations; n = 4. F–O, the PG profiles of RPMI-8226 and U-266 cells challenged by 30 μm SC-560 and NS-398 for 24 h were analyzed by LC/MS/MS. *, p < 0.05 versus vehicle; n = 3. Error bars, S.E.