FIGURE 3.

The TP antagonist inhibited proliferation of two MM cell lines. A–P, proliferation of two MM cell lines incubated with different inhibitors or agonists of PG receptors were analyzed by a CCK-8 assay kit. RPMI-8226 and U-266 cells were treated with graded concentrations of DP2 antagonist, CAY10471 (A and C); EP2 inhibitor, AH-6809 (B and D); EP4 inhibitor, L-161,982 (E and G); TP antagonist, SQ29548 (F and H); DP1 agonist, BW245C (I and K); EP3 agonist, misoprostol (J and L); FP agonist, latanoprost (M and O); or IP agonist, cicaprost (N and P) for 24, 48, 72, and 96 h, respectively. Different colored lines represent different drug concentrations. *, p < 0.05 versus vehicle; #, p < 0.05, differences among drug additional treatments with graded concentrations; n = 3. Error bars, S.E.