FIGURE 8.

The TP antagonist induced RPMI-8226 and U-266 cell apoptosis. A and B, annexin V-FITC/PI staining for apoptosis in two MM cells treated with SC-560 (30 μm), NS-398 (20 μm), SQ29548 (20 μm), and U46619 (20 μm) for 24 h was assessed by flow cytometry analysis. The percentages of early or late apoptosis are presented in the bottom right and top right quadrants, respectively. Columns represent the average proportions of apoptotic cells. *, p < 0.05 versus vehicle; n = 4. C–F, the effects of SC-560, NS-398, SQ29548, and U46619 on caspase 3 activity of two MM cell lines challenged for 24 h were analyzed using a caspase 3 activity assay kit. *, p < 0.05 versus vehicle; n = 3. Error bars, S.E.