TABLE 1.
Primers used in real-time quantitative PCR
| Gene name | Forward primer sequence (5′–3′) | Reverse primer sequence (5′–3′) |
|---|---|---|
| hGAPDH | CATGAGAAGTATGACAACAGCCT | AGTCCTTCCACGATACCAAAGT |
| hCOX-1 | TACTCACAGTGCGCTCCAAC | GCAACTGCTTCTTCCCTTTG |
| hCOX-2 | GCTCAGCCATACAGCAAATCC | CCAAAATCCCCTTGAAGTGGG |
| hDP1 | CTGGGCAAGTGCCTCCTAAG | CAACGAGTTGTCCAATGCGG |
| hDP2 | CCTCTGTGCCCAGAGCCCCACGATG | CACGGCCAAGAAGTAGGTGAAGAAG |
| hEP1 | AGCTTGTCGGTATCATGGTG | AAGAGGCGAAGCAGTTGGC |
| hEP2 | CGATGCTCATGCTCTTCGC | GGGAGACTGCATAGATGACAGG |
| hEP3 | CGCCTCAACCACTCCTACAC | GACACCGATCCGCAATCCTC |
| hEP4 | CCGGCGGTGATGTTCATCTT | CCCACATACCAGCGTGTAGAA |
| hFP | AAGTCCAAGGCATCGTTTCTG | TGACTCCAATACACCGCTCAAT |
| hIP | TTCCGCTTCTACGCCTTCAAC | ACCCAGAGCTTGAGTCGCT |
| hTP | ACGGAGAAGGAGCTGCTCATCT | CCAGCCCCTGAATCCTCA |
| hCDK1 | TGGGAAGTTGGTAGCTCTGAA | AATAAGGCGAAGATCAACATGGC |
| hCyclinB1 | TGGGAAGTTGGTAGCTCTGAA | CCAGGGTGCTTGTCCATGTA |
| hCaspase 3 siRNA | CACAGCACCUGGUUAUUAUTT | AUAAUAACCAGGUGCUGUGTT |
| hCOX-1 siRNA | CGUGAGCUAUUACACUCGUTT | ACGAGUGUAAUAGCUCACGTT |
| hCOX-2 siRNA | GCUCCGGACUAGAUGAUAUTT | AUAUCAUCUAGUCCGGAGCTT |
| hTP siRNA | GGCCCACAAACAUUACCCUTT | AGGGUAAUGUUUGUGGGCCTT |