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. 2015 Dec 30;291(9):4813–4825. doi: 10.1074/jbc.M115.698688

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FIGURE 6. — Induction of IDOL mRNA by DUB inhibition is independent of global LXR activation. A, HepG2 cells cultured in sterol depletion or sterol-rich medium for 18 h. Subsequently, cell were treated with 1 μm GW3965 (6 h), 5 μm HBX41-108, or 50 μm PR-619 (2 h) after which mRNA expression of LDLR, PCSK9, IDOL, LXR, ABCA1, and SREBP-1C were determined by qPCR. Each bar represents the mean ± S.D. relative to vehicle-treated control cells (n = 3). B, human IHH and A431 cell lines and primary human umbilical vein endothelial cells were treated with 5 μm HBX41-108 for 2 h. The mRNA expression of the indicated genes was determined by qPCR. Each bar represents the mean ± S.D. relative to vehicle-treated control cells (n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.001. CTRL, control.