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. 2015 Dec 30;291(9):4813–4825. doi: 10.1074/jbc.M115.698688

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FIGURE 8. — Forced expression of ubiquitin does not prevent IDOL induction by DUB inhibition. A, HepG2 or HepG2-FLAG ubiquitin cells were treated with control or 25 μm MG132 for 2 h. Total cell lysates were immunoblotted as indicated. B, HepG2-FLAG-ubiquitin cells were treated with vehicle (control), 25 μm MG132, 5 μm HBX41-108, or 50 μm PR-619 for 2 h. Subsequently, mRNA expression of Idol was determined by qPCR. Each bar represents the mean ± S.D. relative to vehicle-treated control cells (n = 3). ***, p < 0.001. UB, ubiquitin.