TABLE 2.

Biophysical properties of Ca_v1.2/Ca_vβ3 with Ca_vα2δ1 WT and mutants

Ca_v1.2 WT was co-expressed in stable Ca_vβ3 cells with pmCherry-no insert or pmCherry-Ca_vα2δ1-HA WT or mutant using a 1:1 DNA ratio. Biophysical parameters were measured in the presence of 2 mm Ca²⁺ as described elsewhere (17, 22). Activation properties (E_0.5,|act and ΔG_act) were estimated from the mean I-V relationships and fitted to a Boltzmann equation. The data are shown with the mean ± S.E. of the individual experiments and the number of experiments appears in parentheses. N.D. not determined because of a poor signal to noise ratio. Statistical analysis was carried out against the values obtained in the presence of mCherry-Ca_vα2δ1-HA WT (* p < 0.05; ** p < 0.01).