FIGURE 6.

A, representative images showing a magnified view of eyes with clones from flies of the FRT80B control, dSCCRO^−/− (J34), wild-type control (w1118), and dSCCRO^−/− (J34) with the whole eye clone, FRT42 control, and dSCCRO3^−/− (J155). Control flies maintain an equal proportion of both clones (red and white). In contrast, both dSCCRO^−/− and dSCCRO3^−/− clones (white patch) are smaller than wild-type twin spots (dark red), suggesting that the mutant cells have a defect in proliferation (second panel). Although eye size is normal in dSCCRO^−/− somatic clones, it is markedly reduced in dSCCRO^−/− whole eye clones (compare with w1118 eye). B, representative fluorescent microscopic images showing Caspase 3 (red) and GFP (green) expression in dSCCRO^−/− somatic clones (left) and dSCCRO overexpressing flip out clones (right). Note the presence of apoptotic cells (red) in dSCCRO^−/− somatic GFP-negative clones that border SCCRO wild-type GFP-positive (green) clones. In the dSCCRO overexpression clones (right panel); however, apoptosis (red) is primarily present in GFP-negative cells, SCCRO wild-type cells that border SCCRO-overexpressing GFP-positive cells (green). C, fluorescent microscopic images showing Caspase 3 (red) and GFP (green) expression in dSCCRO4 flip out clones. Apoptotic (red) cells were primarily observed in cells overexpressing dSCCRO4 (GFP-positive cells).