FIGURE 7.

Flux model for PHB and amino acid metabolism in L. pneumophila Paris growing in AYE medium during different growth phases. A, carbon flux in L. pneumophila Paris (Lpp) during the exponential (EE and LE), PE, and late S phase using serine (green arrows) or glucose (yellow arrows) as a substrate. Relative carbon fluxes are indicated by the thickness of the arrows. The citrate cycle is indicated in gray. + to +++, enzymatic activity measured by Keen and Hoffman (30); 1, aconitase; 2, isocitrate dehydrogenase; 3, Glu/Asp transaminase; 4, malate dehydrogenase; 5, malic enzyme (ME; lpp3043 and lpp0705 (5*)). B, carbon flux in L. pneumophila Paris Δketo mutant strain in comparison with the wild type strain measured at the end of the growth phase. The ratios (Δketo mutant/wild type) of incorporation (¹³C mol %) of serine and glucose into selected amino acids or PHB are indicated by red or gray numbers, respectively. *, inactivated lpp1788 (β-ketothiolase). GAP, glyceraldehyde 3-phosphate; ISO, isocitrate; CIT, citrate; KG, ketoglutarate; MAL, malate; OAA, oxaloacetate; TCC, tricarboxylic acid cycle.