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. 2016 Jan 20;291(12):6471–6482. doi: 10.1074/jbc.M115.693481

TABLE 1.

Primers used in this study

Underlined nucleotides indicate the XbaI restriction site.

Name Tm Sequence (5′ → 3′) Ref.
°C
0931-1F GCGAACATTAGGCTTGTCAATA This work
0931-2R GAGATTCAATCATTTTATTGCTCCACT This work
0931-3R2 CATTTCTAGAAATGCCAAATGTTCATC This work
0931-4F GCTTGCTGTCATAAGGAAGTATC This work
iLpp_0650_1U CCGCGGGAATTCGATATCCTTTTAGCCACGATTTACTCCACTT This work
iLpp_0650_2R TAGAAGCTGACATTCTAGCTCCTGAAAGCAAATAATCGAA This work
iLpp_0650_5U TAGACACGATGGCCGTGGATGCCCCAGGGAGTTATGTACT This work
iLpp_0650_6R GAATTCACTAGTGATATCAGCCCTTATTTTAGCCTTTGTTGTC This work
iLpp_0650_3U TGCTTTCAGGAGCTAGAATGTCAGCTTCTAGACTATCTGG This work
iLpp_0650_4R TCCCTGGGGCATCCACGGCCATCGTGTCTAGACACTCCTG This work
iLpp_0650T1U CTTTTAGCCACGATTTACTCCACTT This work
iLpp_0650T6R AGCCCTTATTTTAGCCTTTGTTGTC This work
Lpp_0650_Mut_1U TCAGGTTCGCCTTTTATTGC This work
Lpp_0650_Mut2R AATTCCTGTCCTGCCTTCAG This work
Lpp_0650_Wt_1U CTTTCATCGCTGGTCAGTCA This work
Lpp_0650_Wt_2R ATGAACCGGAGTGTTCCTTG This work
M13R 54.5 GGAAACAGCTATGACCATG 51
M13U 52.8 GTAAAACGACGGCCAGT 51