TABLE 2.

Mutant cycle analyses for interactions at the extracellular terminus of M1^a

^a ΔΔΔG_interaction was calculated from the formula ΔΔΔG_interaction = −RTln((EC₅₀WT · EC₅₀Mut1,2)/(EC₅₀Mut1 · EC₅₀Mut2)) where EC₅₀ = 10^−pH₅₀.

^b Interaction energy was calculated using P119Pip, rather than wild type, as a reference point.