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. 2016 Jan 21;291(12):6232–6244. doi: 10.1074/jbc.M116.713818

FIGURE 3.

FIGURE 3.

Acat1−M/−M does not affect proliferation of hematopoietic progenitor cells in BM. A, LSK, common myeloid progenitor (CMP), granulocyte/macrophage progenitor (GMP), megakaryocyte/erythrocyte-restricted progenitor (MEP), and common lymphoid progenitor (CLP) cells in BM were quantified by flow cytometry as described under “Experimental Procedures.” Results are reported as total number (left panel) and as percentage of total cells within the BM (right panel). 17 WT mice and 10 Acat1−M/−M mice were used for LSK, common myeloid progenitor, megakaryocyte/erythrocyte-restricted progenitor, and granulocyte/macrophage progenitor determinations. B, proliferation of various progenitors was quantified by using bromodeoxyuridine (BrdU) incorporation as described under “Experimental Procedures.” C, BM B (CD19+), pro-B (CD43+CD19+), and pre-B (CD43−CD19+IgM−) cells were measured as total cell number (left panel) and as percentage of total cells within the BM (right panel) as described under “Experimental Procedures.” Proliferation of B and B progenitor cells was quantified by bromodeoxyuridine. Results are reported as mean ± S.E. Mice fed a chow diet were analyzed when they were 2 months old.