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The effect of the M4 segment on tetramerization with or without the ECD. A, VLGAVE face (dark gray) of the M4 segment in the GluA2 structure of Protein Data Bank 3KG2 (6). Numbering is for GluA1. B, BN-PAGE of GluA1 and mutants with tryptophan (W) substitutions in the VLGAVE face (19). C and D, BN-PAGE of GluA1-ΔATD (C), HA-GluA1-ΔECD or HA-GluA1-ΔECD-EGFP (D) with Trp substitutions in the VLGAVE face.
