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. 2016 Jan 21;291(12):6396–6411. doi: 10.1074/jbc.M115.686840

FIGURE 2.

FIGURE 2.

Ty1-IN interacts with RNA Pol III subunits. A, immunoblot of whole cell lysate (Lysate) and GFP-Trap IP carried out from Rpc37-GFP, Rpc53-GFP, Rpc82-GFP, Maf1-GFP, Rpc17-GFP, and untagged wild type (No Tag) cells. B, immunoblot of whole cell lysate (Lysate) and GFP-Trap IP carried out from Rpc19-GFP, Rpc40-GFP, Rpb6-GFP, and untagged wild type (No Tag) cells. C, immunoblot of whole cell lysate (Lysate) and anti-HA IP performed from Rpc82-3HA, Rpc34-3HA, Rpc25-3HA, and untagged wild type (No Tag) cells. The asterisk marks the heavy chain antibody. For A–C, expression of a Ty1 element (pGAL1-Ty1-H3, pJEF724) was induced in all strains for 24 h prior to cell lysis. Immunoprecipitates were washed with either a high salt (HS; 500 mm NaCl) or low salt (LS; 300 mm NaCl) wash. Immunoblots were probed with anti-GFP (A and B), anti-HA (C), and anti-IN (8b11) (A–C) antibodies.