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The interaction between Ty1-IN and Rpc37 is disrupted in the rpc53Δ2–280 mutant. An immunoblot of whole cell lysate (Lysate) and GFP-Trap IP carried out from untagged (No Tag), Rpc17-GFP, Rpc19-GFP, Rpc37-GFP, and Rpc82-GFP cell lysates in a wild type or rpc53Δ2–280 (Δ) strain background. Expression of a Ty1 element (pGAL1-Ty1-H3, pJEF724) was induced in all strains for 24 h prior to cell lysis. Immunoblots were probed with anti-GFP and anti-IN (8b11) antibodies.
