FIGURE 6.
Regulatory profiles and localizations of Mit1 and Prm1 in different carbon sources. A, transcription level of PRM1 and expression of GFP driven by PPRM1 in WT cells cultured in glucose, glycerol, methanol, and ethanol. B, transcription level of MIT1 and expression of GFP driven by PMIT1 in the WT cells cultured in glucose, glycerol, methanol, and ethanol. For A and B, the mRNA levels were normalized to the levels of the mRNA of housekeeping gene ACT1 in each sample. The relative expression level indicated on the y axis (2−ΔΔCT) for each gene at different carbon sources was normalized for its expression in glucose-grown cells. The error bars represent the standard deviation of three biological replicates, each with three technical replicates, assayed in duplicate. An independent-sample t test was used to determine the statistical significance of the glycerol, methanol, and ethanol groups relative to glucose groups in the corresponding mRNA or GFP assay. mRNA: **, p < 0.01. GFP: ‡, p < 0.05; ‡‡, p < 0.01. C and D, localization of GFP-Prm1 (C) and GFP-Mit1 (D) in glucose, glycerol, and methanol. DAPI was used to stain the cell nucleus. DIC, differential interference contrast.