Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
FakB2(R202A) exhibits a lower affinity for FakA. The standard radiochemical Fak assay described under “Experimental Procedures” was used to compare the apparent Km values for FakB2 and FakB2(R202A). The apparent Km value for FakB2 was 0.16 ± 0.02 μm, and the apparent Km value for FakB2(R202A) was 1.67 ± 0.17 μm. The Km values were calculated using non-linear Michaelis-Menten regression analysis using GraphPad software.
