TABLE 3.

Sedimentation velocity c(s) analysis of FakA, FakB2, and the mixtures of FakA and FakB2 (and its mutants)

Sample	μma	mg/mlb	s20 (Svedberg)c	s20, w (Svedberg)d
FakA	122.0	1.15	5.59 (88%)	5.86
FakB2	231.0	1.10	2.64 (98%)	2.79
FakB2 + FakA	18.91 + 7.85	1.23	2.50 (29%)	2.62
			6.79 (61%)	7.15
FakB2(T61A) + FakA	16.37 + 8.19	1.15	2.76 (23%)	2.90
			6.68 (67%)	7.04
FakB2(S93A) + FakA	14.23 + 7.85	1.08	2.76 (20%)	2.90
			6.64 (70%)	7.07
FakB2(R170A) + FakA	19.68 + 7.91	1.17	2.60 (33%)	2.73
			6.70 (65%)	7.01
FakB2(R202A) + FakA	19.89 + 4.39	1.19	2.64 (51%)	2.78
			5.67 (43%)	5.97
FakB2(H226A) + FakA	19.60 + 8.01	1.22	2.56 (31%)	2.70
			6.72 (63%)	7.08

^a Total loading concentrations in μm of FakB2 and its mutants (left-hand side) and FakA on the right in 20 mm Tris, pH 7.5, 0.2 m NaCl at 20 °C.

^b Total concentration is shown in mg/ml.

^c Sedimentation coefficient taken from the ordinate maximum of each peak in the best fit c(s) distribution at 20 °C with percentage protein amount in parentheses. Sedimentation coefficient (s-value) is a measure of the size and shape of a protein in a solution with a specific density and viscosity at a specific temperature.

^d Standard sedimentation coefficient (s_{20, w} value) in water at 20 °C.